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Storkbio Ltd
Kämmu, Kupu, 74610 Kuusalu, Estonia
Phone +372 6604793
www.storkbio.com
info@storkbio.com
 
Migration Assay
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Cell migration has two major components: directional migration and random migration. Directional migration, or chemotaxis, is exhibited when cells detect and move along the chemoattractant gradient. Cell migration has been examined by a quantitative method developed by Boyden. In the Boyden assay two chambers are separated by a microporous membrane. A cell suspension is loaded into one chamber, and medium supplemented with the chemoattractant is placed into the other chamber, forming a very steep gradient. The cells migrate toward the chemoattractant through the membrane, and the migrated cells are quantified. The migrated cells can be quantified by counting the fixed and stained cells on the bottom side of the microporous membrane or by measurement of total well fluorescence.  

 

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